                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  HNL mutants at low pH

<130>  Case 29306

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  532
<212>  PRT
<213>  Prunus amygdalus

<400>  1

Leu Ala Asn Thr Ser Ala His Asp Phe Ser Tyr Leu Lys Phe Val Tyr 
1               5                   10                  15      


Asn Ala Thr Asp Thr Ser Ser Glu Gly Ser Tyr Asp Tyr Ile Val Ile 
            20                  25                  30          


Gly Gly Gly Thr Ser Gly Cys Pro Leu Ala Ala Thr Leu Ser Glu Lys 
        35                  40                  45              


Tyr Lys Val Leu Leu Leu Glu Arg Gly Thr Ile Ala Thr Glu Tyr Pro 
    50                  55                  60                  


Asn Thr Leu Thr Ala Asp Gly Phe Ala Tyr Asn Leu Gln Gln Gln Asp 
65                  70                  75                  80  


Asp Gly Lys Thr Pro Val Glu Arg Phe Val Ser Glu Asp Gly Ile Asp 
                85                  90                  95      


Asn Val Arg Ala Arg Ile Leu Gly Gly Thr Thr Ile Ile Asn Ala Gly 
            100                 105                 110         


Val Tyr Ala Arg Ala Asn Ile Ser Phe Tyr Ser Gln Thr Gly Ile Glu 
        115                 120                 125             


Trp Asp Leu Asp Leu Val Asn Lys Thr Tyr Glu Trp Val Glu Asp Ala 
    130                 135                 140                 


Ile Val Val Lys Pro Asn Asn Gln Ser Trp Gln Ser Val Ile Gly Glu 
145                 150                 155                 160 


Gly Phe Leu Glu Ala Gly Ile Leu Pro Asp Asn Gly Phe Ser Leu Asp 
                165                 170                 175     


His Glu Ala Gly Thr Arg Leu Thr Gly Ser Thr Phe Asp Asn Asn Gly 
            180                 185                 190         


Thr Arg His Ala Ala Asp Glu Leu Leu Asn Lys Gly Asp Pro Asn Asn 
        195                 200                 205             


Leu Leu Val Ala Val Gln Ala Ser Val Glu Lys Ile Leu Phe Ser Ser 
    210                 215                 220                 


Asn Thr Ser Asn Leu Ser Ala Ile Gly Val Ile Tyr Thr Asp Ser Asp 
225                 230                 235                 240 


Gly Asn Ser His Gln Ala Phe Val Arg Gly Asn Gly Glu Val Ile Val 
                245                 250                 255     


Ser Ala Gly Thr Ile Gly Thr Pro Gln Leu Leu Leu Leu Ser Gly Val 
            260                 265                 270         


Gly Pro Glu Ser Tyr Leu Ser Ser Leu Asn Ile Thr Val Val Gln Pro 
        275                 280                 285             


Asn Pro Tyr Val Gly Gln Phe Val Tyr Asp Asn Pro Arg Asn Phe Ile 
    290                 295                 300                 


Asn Ile Leu Pro Pro Asn Pro Ile Glu Ala Ser Val Val Thr Val Leu 
305                 310                 315                 320 


Gly Ile Arg Ser Asp Tyr Tyr Gln Val Ser Leu Ser Ser Leu Pro Phe 
                325                 330                 335     


Ser Thr Pro Pro Phe Ser Leu Phe Pro Thr Thr Ser Tyr Pro Leu Pro 
            340                 345                 350         


Asn Ser Thr Phe Ala His Ile Val Ser Gln Val Pro Gly Pro Leu Ser 
        355                 360                 365             


His Gly Ser Val Thr Leu Asn Ser Ser Ser Asp Val Arg Ile Ala Pro 
    370                 375                 380                 


Asn Ile Lys Phe Asn Tyr Tyr Ser Asn Ser Thr Asp Leu Ala Asn Cys 
385                 390                 395                 400 


Val Ser Gly Met Lys Lys Leu Gly Asp Leu Leu Arg Thr Lys Ala Leu 
                405                 410                 415     


Glu Pro Tyr Lys Ala Arg Asp Val Leu Gly Ile Asp Gly Phe Asn Tyr 
            420                 425                 430         


Leu Gly Val Pro Leu Pro Glu Asn Gln Thr Asp Asp Ala Ser Phe Glu 
        435                 440                 445             


Thr Phe Cys Leu Asp Asn Val Ala Ser Tyr Trp His Tyr His Gly Gly 
    450                 455                 460                 


Ser Leu Val Gly Lys Val Leu Asp Asp Ser Phe Arg Val Met Gly Ile 
465                 470                 475                 480 


Lys Ala Leu Arg Val Val Asp Ala Ser Thr Phe Pro Tyr Glu Pro Asn 
                485                 490                 495     


Ser His Pro Gln Gly Phe Tyr Leu Met Leu Gly Arg Tyr Val Gly Leu 
            500                 505                 510         


Gln Ile Leu Gln Glu Arg Ser Ile Arg Leu Glu Ala Ile His Asn Ile 
        515                 520                 525             


Gln Glu Ser Met 
    530         


<210>  2
<211>  532
<212>  PRT
<213>  Artificial sequence

<220>
<223>  modified PaHNL5

<400>  2

Leu Ala Asn Thr Ser Ala His Asp Phe Ser Tyr Leu Lys Phe Val Tyr 
1               5                   10                  15      


Asn Ala Thr Asp Thr Ser Ser Glu Gly Ser Tyr Asp Tyr Ile Val Ile 
            20                  25                  30          


Gly Gly Gly Thr Ser Gly Cys Pro Leu Ala Ala Thr Leu Ser Glu Lys 
        35                  40                  45              


Tyr Lys Val Leu Leu Leu Glu Arg Gly Thr Ile Ala Thr Glu Tyr Pro 
    50                  55                  60                  


Asn Thr Leu Thr Ala Asp Gly Phe Ala Tyr Asn Leu Gln Gln Gln Asp 
65                  70                  75                  80  


Asp Gly Lys Thr Pro Val Glu Arg Phe Val Ser Glu Asp Gly Ile Asp 
                85                  90                  95      


Asn Val Arg Ala Arg Ile Leu Gly Gly Thr Thr Ile Ile Asn Ala Gly 
            100                 105                 110         


Val Tyr Ala Arg Ala Asn Ile Ser Phe Tyr Ser Gln Thr Gly Ile Glu 
        115                 120                 125             


Trp Asp Leu Asp Leu Val Asn Lys Thr Tyr Glu Trp Val Glu Asp Ala 
    130                 135                 140                 


Ile Val Val Lys Pro Asn Asn Gln Ser Trp Gln Ser Val Ile Gly Glu 
145                 150                 155                 160 


Gly Phe Leu Glu Ala Gly Ile Leu Pro Asp Asn Gly Phe Ser Leu Asp 
                165                 170                 175     


His Glu Ala Gly Thr Arg Leu Thr Gly Ser Thr Phe Asp Asn Asn Gly 
            180                 185                 190         


Thr Arg His Ala Ala Asp Glu Leu Leu Asn Lys Gly Asp Pro Asn Asn 
        195                 200                 205             


Leu Leu Val Ala Val Gln Ala Ser Val Glu Lys Ile Leu Phe Ser Ser 
    210                 215                 220                 


Asn Thr Ser Asn Leu Ser Ala Ile Gly Val Ile Tyr Thr Asp Ser Asp 
225                 230                 235                 240 


Gly Asn Ser His Gln Ala Phe Val Arg Gly Asn Gly Glu Val Ile Val 
                245                 250                 255     


Ser Ala Gly Thr Ile Gly Thr Pro Gln Leu Leu Leu Leu Ser Gly Val 
            260                 265                 270         


Gly Pro Glu Ser Tyr Leu Ser Ser Leu Asn Ile Thr Val Val Gln Pro 
        275                 280                 285             


Asn Pro Tyr Val Gly Gln Phe Val Tyr Asp Asn Pro Arg Asn Phe Ile 
    290                 295                 300                 


Asn Ile Leu Pro Pro Asn Pro Ile Glu Ala Ser Val Ala Thr Val Leu 
305                 310                 315                 320 


Gly Ile Arg Ser Asp Tyr Tyr Gln Val Ser Leu Ser Ser Leu Pro Phe 
                325                 330                 335     


Ser Thr Pro Pro Phe Ser Leu Phe Pro Thr Thr Ser Tyr Pro Leu Pro 
            340                 345                 350         


Asn Ser Thr Phe Ala His Ile Val Ser Gln Val Pro Gly Pro Leu Ser 
        355                 360                 365             


His Gly Ser Val Thr Leu Asn Ser Ser Ser Asp Val Arg Ile Ala Pro 
    370                 375                 380                 


Asn Ile Lys Phe Asn Tyr Tyr Ser Asn Ser Thr Asp Leu Ala Asn Cys 
385                 390                 395                 400 


Val Ser Gly Met Lys Lys Leu Gly Asp Leu Leu Arg Thr Lys Ala Leu 
                405                 410                 415     


Glu Pro Tyr Lys Ala Arg Asp Val Leu Gly Ile Asp Gly Phe Asn Tyr 
            420                 425                 430         


Leu Gly Val Pro Leu Pro Glu Asn Gln Thr Asp Asp Ala Ser Phe Glu 
        435                 440                 445             


Thr Phe Cys Leu Asp Asn Val Ala Ser Tyr Trp His Tyr His Gly Gly 
    450                 455                 460                 


Ser Leu Val Gly Lys Val Leu Asp Asp Ser Phe Arg Val Met Gly Ile 
465                 470                 475                 480 


Lys Ala Leu Arg Val Val Asp Ala Ser Thr Phe Pro Tyr Glu Pro Ser 
                485                 490                 495     


Ser His Pro Gln Gly Phe Tyr Leu Met Leu Gly Arg Tyr Val Gly Leu 
            500                 505                 510         


Gln Ile Leu Gln Glu Arg Ser Ile Arg Leu Glu Ala Ile His Asn Ile 
        515                 520                 525             


Gln Glu Ser Met 
    530         


